Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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Validation data
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- Product number
- PA5-51470 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FKBP25 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LPKKDIIKFL QEHGSDSFLA EHKLLGNIKN VAKTANKDHL VTAYNHLFET KRFKGTESIS KVSEQVKNVK LNEDKPKETK SEETLDEGPP KYTKSVLKKG DKTNFPKKGD VVHCWYTGTL QDGT Highest antigen sequence identity to the following orthologs: Mouse - 92%, Rat - 94%.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FKBP25 in Lane 1: Marker (kDa) 230, 110, 82, 49, 32, 26, 18; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using a FKBP25 Polyclonal Antibody (Product # PA5-51470).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FKBP25 in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells); Lane 3: PC12 cell lysate (Pheochromocytoma of rat adrenal medulla). Samples were probed using a FKBP25 Polyclonal Antibody (Product # PA5-51470).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FKBP25 in human cell line U-2 OS shows positivity in cytoplasm. Samples were probed using a FKBP25 Polyclonal Antibody (Product # PA5-51470).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of FKBP25 in human testis using FKBP25 Polyclonal Antibody (Product # PA5-51470) shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of FKBP25 in human lymphoid tissues using FKBP25 Polyclonal Antibody (Product # PA5-51470) shows moderate to strong cytoplasmic positivity in germinal center cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of FKBP25 in human skeletal muscle using FKBP25 Polyclonal Antibody (Product # PA5-51470) shows moderate cytoplasmic positivity in myocytes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of FKBP25 in human cerebral cortex using FKBP25 Polyclonal Antibody (Product # PA5-51470) shows moderate to strong cytoplasmic positivity in neurons.