PA5-60958

Antibody data

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Validation data

Product number: PA5-60958
Provider: Invitrogen Antibodies
Product name: Anti-COL6A6 Polyclonal Antibody
Provider product page: Invitrogen Antibodies - PA5-60958
Antibody type: Polyclonal
Antigen: Recombinant full-length protein
Description: Immunogen sequence: ALREVEHYFR PDMGSRINTG TPQVLLVLTD GQSQDEVAQA AEALRHRGID IYSVGIGDVD DQQLIQITGT AEKKLTVHNF DELKKVNKRI VRN Highest antigen sequence identity to the following orthologs: Mouse - 87%, Rat - 90%.
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 5 Collagen protein expression in HNSCC patients' tissues. COL3A1 and COL6A6 protein expression was evaluated in CAF-P ( A ) and CAF-D ( B ) tissues from patients with HNSCC.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 The expression of COL6A6 and P4HA3 in PA tissues and cell lines. ( A and B ) RT-qPCR was used to detect the expression of COL6A6 and P4HA3 in PA tissues and normal pituitary tissues; ( C and D ) Immunohistochemistry staining was applied to detect the expression of COL6A6 and P4HA3 in PA tissues and normal pituitary tissues; ( E and F ) Western blotting was used to evaluate the expression of COL6A6 and P4HA3 in PA cell lines. *** P

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3 The effect of COL6A6 on the proliferation, invasion, migration, and EMT of AtT-20 and HP75 cells . ( A ) The expression of COL6A6 protein was determined by western blotting; ( B ) The proliferation ability of AtT-20 and HP75 cells were measured by CCK-8 assay; ( C and D ) The invasion of AtT-20 and HP75 cells were measured by Transwell assay (40x); ( E - G ) The migration ability of AtT-20 and HP75 cells were evaluated by wound healing assay (4x); ( H - K ) The expression of EMT related proteins in AtT-20 and HP75 cells were detected by western blotting. * P

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 4 The effect of COL6A6 on the growth and metastasis of xenograft tumor . ( A and B ) The tumor size was obtained from nude mice; ( C and D ) Tumor volume and tumor weight of nude mice were analyzed; ( E and F ) The expression of COL6A6 in xenograft tumor tissues were detected by western blotting; ( G and H ) The expression of Ki-67 in xenograft tumor tissues were detected by immunohistochemistry staining; ( I and J ) The expression of E-cadherin, vimentin, and N-cadherin were measured by western blotting. ** P

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 5 Effect of COL6A6 on the PI3K-Akt signaling pathway through interacting with P4HA3 . ( A and B ) Western blotting was used to detect the expression of COL6A6, P4HA3, PI3K-Akt pathway-related protein in AtT-20 and HP75 cells; ( C and D ) Coimmunoprecipitation to validate the COL6A6-P4HA3 interaction. ** P