Antibody data
- Product number
- PA5-60958
- Provider
- Invitrogen Antibodies
- Product name
- Anti-COL6A6 Polyclonal Antibody
- Provider product page
- Invitrogen Antibodies - PA5-60958
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: ALREVEHYFR PDMGSRINTG TPQVLLVLTD GQSQDEVAQA AEALRHRGID IYSVGIGDVD DQQLIQITGT AEKKLTVHNF DELKKVNKRI VRN Highest antigen sequence identity to the following orthologs: Mouse - 87%, Rat - 90%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Cancer-Associated Fibroblast Subgroups Showing Differential Promoting Effect on HNSCC Progression.
Kang SH, Oh SY, Lee HJ, Kwon TG, Kim JW, Lee ST, Choi SY, Hong SH
Cancers 2021 Feb 6;13(4)
COL6A6 interacted with P4HA3 to suppress the growth and metastasis of pituitary adenoma via blocking PI3K-Akt pathway.
Long R, Liu Z, Li J, Yu H
Aging 2019 Oct 17;11(20):8845-8859
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image

- Experimental details
- Immunohistochemical staining of COL6A6 in human lung using COL6A6 Polyclonal Antibody (Product # PA5-60958) shows moderate to strong membranous positivity in pneumocytes.
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image

- Experimental details
- Immunohistochemical staining of COL6A6 in human skin using COL6A6 Polyclonal Antibody (Product # PA5-60958) shows moderate positivity in extracellular matrix.
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image

- Experimental details
- Immunohistochemical staining of COL6A6 in human breast using COL6A6 Polyclonal Antibody (Product # PA5-60958) shows moderate positivity in extracellular matrix.
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image

- Experimental details
- Immunohistochemical staining of COL6A6 in human pancreas using COL6A6 Polyclonal Antibody (Product # PA5-60958) shows very weak positivity in exocrine glandular cells.
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image

- Experimental details
- Figure 5 Collagen protein expression in HNSCC patients' tissues. COL3A1 and COL6A6 protein expression was evaluated in CAF-P ( A ) and CAF-D ( B ) tissues from patients with HNSCC.
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image

- Experimental details
- Figure 2 The expression of COL6A6 and P4HA3 in PA tissues and cell lines. ( A and B ) RT-qPCR was used to detect the expression of COL6A6 and P4HA3 in PA tissues and normal pituitary tissues; ( C and D ) Immunohistochemistry staining was applied to detect the expression of COL6A6 and P4HA3 in PA tissues and normal pituitary tissues; ( E and F ) Western blotting was used to evaluate the expression of COL6A6 and P4HA3 in PA cell lines. *** P
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image

- Experimental details
- Figure 3 The effect of COL6A6 on the proliferation, invasion, migration, and EMT of AtT-20 and HP75 cells . ( A ) The expression of COL6A6 protein was determined by western blotting; ( B ) The proliferation ability of AtT-20 and HP75 cells were measured by CCK-8 assay; ( C and D ) The invasion of AtT-20 and HP75 cells were measured by Transwell assay (40x); ( E - G ) The migration ability of AtT-20 and HP75 cells were evaluated by wound healing assay (4x); ( H - K ) The expression of EMT related proteins in AtT-20 and HP75 cells were detected by western blotting. * P
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image

- Experimental details
- Figure 4 The effect of COL6A6 on the growth and metastasis of xenograft tumor . ( A and B ) The tumor size was obtained from nude mice; ( C and D ) Tumor volume and tumor weight of nude mice were analyzed; ( E and F ) The expression of COL6A6 in xenograft tumor tissues were detected by western blotting; ( G and H ) The expression of Ki-67 in xenograft tumor tissues were detected by immunohistochemistry staining; ( I and J ) The expression of E-cadherin, vimentin, and N-cadherin were measured by western blotting. ** P
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image

- Experimental details
- Figure 5 Effect of COL6A6 on the PI3K-Akt signaling pathway through interacting with P4HA3 . ( A and B ) Western blotting was used to detect the expression of COL6A6, P4HA3, PI3K-Akt pathway-related protein in AtT-20 and HP75 cells; ( C and D ) Coimmunoprecipitation to validate the COL6A6-P4HA3 interaction. ** P