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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-34856 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CUG-BP2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: PC-3, U87-MG, mouse brain. Predicted reactivity: Mouse (90%), Rat (89%), Zebrafish (85%), Xenopus laevis (91%), Chicken (90%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CUG-BP2 Polyclonal Antibody (Product # PA5-34856). Sample (30 µg of whole cell lysate). Lane A: PC-3. Lane B: U87-MG . 7.5% SDS PAGE. CUG-BP2 Polyclonal Antibody (Product # PA5-34856) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CUG-BP2 Polyclonal Antibody (Product # PA5-34856). Sample (50 µg of whole cell lysate). Lane A: mouse brain . 7.5% SDS PAGE. CUG-BP2 Polyclonal Antibody (Product # PA5-34856) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CUG-BP2 Polyclonal Antibody detects CUGBP2 protein at nucleus by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CUGBP2 protein stained by CUG-BP2 Polyclonal Antibody (Product # PA5-34856) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of CUG-BP2 was performed in paraffin-embedded mouse brain tissue using CUG-BP2 Polyclonal Antibody (Product # PA5-34856) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft, using CUGBP2 (Product # PA5-34856) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
