PA5-117867

CHMP4A antibody from Invitrogen Antibodies

C14orf123, HSPC134, VPS32A

Western blot

Supportive data in Antibodypedia

Immunoprecipitation

Data presented on provider website

Immunohistochemistry

Supportive data in Antibodypedia

Other assay

Supportive data in Antibodypedia

Provider product page for PA5-117867

Antibody Data

Product number

PA5-117867

Provider

Invitrogen Antibodies

Product name

Anti-CHMP4A Polyclonal Antibody

Provider product page

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using CHMP4A Polyclonal Antibody (Product # PA5-117867) at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate, Lane B: RT4 Whole Cell Lysate, Lane C: HL60 Whole Cell Lysate, Lane D: MOLT4 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 25 kDa. Observed band size: 32 kDa.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human CHMP4A in human kidney with CHMP4A Polyclonal Antibody (Product # PA5-117867, 1:100 dilution, formalin-fixed paraffin embedded sections).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human CHMP4A in human rectum with CHMP4A Polyclonal Antibody (Product # PA5-117867, 1:100 dilution, formalin-fixed paraffin embedded sections).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CHMP4A Immunoprecipitation using: Lane A: 0.5 mg HepG2 Whole Cell Lysate 4 µL with CHMP4A Polyclonal Antibody (Product # PA5-117867) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: CHMP4A Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 25 kDa. Observed band size: 32 kDa.