PA5-49392

antibody from Invitrogen Antibodies

Targeting: WDR82 MST107, MSTP107, PRO2730, PRO34047, SWD2, TMEM113, WDR82A

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-49392

Provider

Invitrogen Antibodies

Product name

WDR82 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Predicted to react with xenopus and chicken based on sequence homology.

Reactivity

Human, Mouse, Zebrafish

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of WDR82 in various lysates. Samples were incubated with WDR82 polyclonal antibody (Product # PA5-49392) using a dilution of 1:1,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Lane 1: F9 whole cell lysate; Lane 2:NIH/3T3 whole cell lysate; Lane 3: SH-SY5Y whole cell lysate. Predicted band size: 35 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of WDR82 in paraformaldehyde-fixed, paraffin-embedded human brain tissue sections. Samples were incubated with WDR82 polyclonal antibody (Product # PA5-49392) using a dilution of 1:250 for 1 hours at room temperature followed by an undiluted biotinylated goat polyvalent antibody.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of (overlay histogram) of WDR82 in HepG2 cells (green line). Samples were incubated with WDR82 polyclonal antibody (Product # PA5-49392) using a dilution of 1:25 dilution for 60 min at 37°C followed by Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1:200 dilution for 40 min at 37°C. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the primary antibody. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.