M00067-1

antibody from Boster Biological Technology

Targeting: INS IDDM1, IDDM2

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

M00067-1

Provider

Boster Biological Technology

Product name

Anti-Insulin Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for Insulin/INS detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IF, IHC, ICC. Reactive species: Human, Mouse, Rat Insulin/INS information: Molecular Weight: 11981 MW; Subcellular Localization: Secreted.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Antibody clone number

DEA-9

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Immunofluorescent analysis of BxPC-3 cells, using Insulin Antibody (M00067-1). Insulin was detected in immunocytochemical section. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti- Insulin Antibody (M00067-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Additional image