MAB6088

antibody from Novus Biologicals

Targeting: NLGN3 ASPGX1, AUTSX1, HNL3, KIAA1480

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB6088

Provider

Novus Biologicals

Product name

Mouse Monoclonal Neuroligin 3/NLGN3 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human Neuroligin 3 in direct ELISAs. In this format, 100% cross-reactivity with recombinant human (rh) Neuroligin 3 variant 2 and recombinant rat Neuroligin 3 variant 2 and no cross-reactivity with rhNeuroligin 1 variant 2, rhNeuroligin 2, rhNeuroligin 4, recombinant rat Neuroligin 1, 1 variant 2, 2 variant 2, or 3 variant 4 is observed.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Global Analysis of Protein Expression of Inner Ear Hair Cells.

Hickox AE, Wong AC, Pak K, Strojny C, Ramirez M, Yates JR 3rd, Ryan AF, Savas JN
The Journal of neuroscience : the official journal of the Society for Neuroscience 2017 Feb 1;37(5):1320-1339

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Neuroligin 3/NLGN3 in Human Brain. Neuroligin 3/NLGN3 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Human Neuroligin 3/NLGN3 Monoclonal Antibody (Catalog # MAB6088) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.