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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [1]
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- Product number
- MA5-24253 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NLGN3 Monoclonal Antibody (566209)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- 100% cross-reactivity with recombinant human (rh) Neuroligin 3 variant 2 and recombinant rat Neuroligin 3 variant 2 and no cross-reactivity with rhNeuroligin 1 variant 2, rhNeuroligin 2, rhNeuroligin 4, recombinant rat Neuroligin 1, 1 variant 2, 2 variant 2, or 3 variant 4 is observed. Reconstitute at 0.5 mg/mL in sterile PBS.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 566209
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Synaptic protein interaction networks encode experience by assuming stimulus-specific and brain-region-specific states.
Lautz JD, Tsegay KB, Zhu Z, Gniffke EP, Welsh JP, Smith SEP
Cell reports 2021 Nov 30;37(9):110076
Cell reports 2021 Nov 30;37(9):110076
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemical analysis of NLGN3 in immersion fixed paraffin-embedded sections of human brain (cerebellum). Samples were incubated in NLGN3 monoclonal antibody (Product # MA5-24253) using a dilution of 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents.
