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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-78508 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NLGN3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: mouse brain, rat brain, SK-N-SH
- Concentration
- 1.41 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NLGN3 in whole cell lysate using 30 µg of protein. Samples were separated with 7.5% SDS-PAGE and incubated with NLGN3 polyclonal antibody (Product # PA5-78508) using a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NLGN3 in various tissue extracts using 50 µg of protein. Samples were separated with 7.5% SDS-PAGE and incubated with NLGN3 polyclonal antibody (Product # PA5-78508) using a dilution of 1:10,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NLGN3 was performed by loading 15ug dorsal hippocampal lysate from NLGN3-/- mice (KO) and wildtype mice (WT). NLGN3 was detected at approximately 110kDa by using a NLGN3 Polyclonal Antibody (Product # PA5-78508) at a dilution of 1:100 in Blocking Buffer overnight at 4°C on a rocking platform, followed by a 1-hour room-temperature incubation of a donkey anti-rabbit IgG antibody at a dilution of 1:5000. Data courtesy of Thermo Scientific KOL Program.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NLGN3 Polyclonal Antibody (Product # PA5-78508). Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Neuroligin 3 NLGN3 Polyclonal Antibody (Product # PA5-78508) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NLGN3 Polyclonal Antibody (Product # PA5-78508). Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NLGN3 Polyclonal Antibody (Product # PA5-78508) diluted at 1:10,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NLGN3 Polyclonal Antibody detects Neuroligin 3 protein by immunofluorescent analysis. Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: Neuroligin 3 protein stained by NLGN3 Polyclonal Antibody (Product # PA5-78508) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta-3 Tubulin Polyclonal Antibody [GT1338]diluted at 1:500. Blue: Fluoroshield with DAPI .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NLGN3 Polyclonal Antibody detects Neuroligin 3 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. NLGN3 Polyclonal Antibody (Product # PA5-78508) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
