A304-809A

antibody from Invitrogen Antibodies

Targeting: WASHC5 KIAA0196, SPG8

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

A304-809A

Provider

Invitrogen Antibodies

Product name

SPG8/Strumpellin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human SPG8/Strumpellin by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SPG8/Strumpellin antibody A304-809A (lot A304-809A-1) used for IP at 6 µg per reaction. SPG8/Strumpellin was inefficiently immunoprecipitated by rabbit anti-SPG8/Strumpellin antibody BL18606. For blotting immunoprecipitated SPG8/Strumpellin, A304-809A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse SPG8/Strumpellin by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SPG8/Strumpellin antibody A304-809A (lot A304-809A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.