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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- A305-821A-T - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- OTULIN Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 10 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse OTULIN by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, U2OS, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-OTULIN antibody A305-821A-M (lot A305-821A-M-1) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human OTULIN by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-OTULIN antibody (Product # A305-821A-T; lot A305-821A-T-1) used for IP at 40 µL per reaction. OTULIN was also immunoprecipitated by rabbit anti-OTULIN antibody (Product # A305-822A-T). For blotting immunoprecipitated OTULIN, Product # A305-821A-T was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.
