Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- F55036 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Thyroxine 5-deiodinase Antibody / DIO3
- Antibody type
- Polyclonal
- Description
- This highly specific Thyroxine 5-deiodinase antibody is suitable for use in Western blot/Immunohistochemistry/Immunohistochemistry applications with human and rat samples.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 0.08 ml, 0.4 ml
- Concentration
- In 1X PBS, pH 7.4, with 0.09% sodium azide
- Storage
- Aliquot the Thyroxine 5-deiodinase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human placental tissue lysate with Thyroxine 5-deiodinase antibody. Predicted molecular weight ~34 kDa, routinely observed at 31-37 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human HepG2 cell lysate with Thyroxine 5-deiodinase antibody. Predicted molecular weight ~34 kDa, routinely observed at 31-37 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of rat skeletal muscle tissue lysate with Thyroxine 5-deiodinase antibody. Predicted molecular weight ~34 kDa, routinely observed at 31-37 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human hepatocellular carcinoma tissue with Thyroxine 5-deiodinase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.