NBP2-16953

antibody from Novus Biologicals

Targeting: IL3RA CD123

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-16953

Provider

Novus Biologicals

Product name

Rabbit Polyclonal IL-3R alpha/CD123 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: IL-3 R alpha/CD123 Antibody [NBP2-16953] - IL3 Receptor alpha antibody [N2C2], Internal detects IL3 Receptor alpha protein by western blot analysis. THP-1 whole cell extracts (30 ug), non-transfected (-) and IL3 Receptor alpha-transfected (+) 293T whole cell extracts (5 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IL3 Receptor alpha antibody diluted by 1:1000

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: IL-3 R alpha/CD123 Antibody [NBP2-16953] - Human breast cancer, using IL3 Receptor alpha antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: IL-3 R alpha/CD123 Antibody [NBP2-16953] - Human esophageal cancer. IL3 Receptor alpha antibody [N2C2], Internal diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min