Antibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- BS60620 - Provider product page
- Provider
- Bioworld Technology, Inc
- Product name
- IL3RA polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- A synthetic peptide corresponding to residues in Human IL3RA
- Description
- Interleukin-3, or IL-3, is a pleiotropic cytokine that is primarily secreted by activated T lymphocytes and stimulates the proliferation and differentiation of hematopoietic cells. IL-3 exerts its biological effects through a receptor which consists of a ligand-specific Ï« subunit (IL-3RÏ«) and a signal transducing ÉÇ subunit (IL-3RÉÇ) common to the IL-3/IL-5/GM-CSF receptors. The Ï« subunits are low-affinity ligand-binding proteins while the ÉÇ subunits do not themselves bind ligand, but are required for high affinity binding by the Ï« subunits. The mouse IL-3 receptor has two distinct ÉÇ subunits, one that functions only in IL-3-mediated cell signaling and a second that is shared with IL-5 and GM-CSF. The murine ÉÇ subunits are 91% homologous at the amino acid level but only 56% homologous to the human ÉÇ subunit. The carboxy-terminus of the ÉÇ subunit has been shown to be necessary for activation of the MAP kinase signaling pathway. Although the IL-3 receptor has no intrinsic kinase activity, stimulation with IL-3 leads to tyrosine phosphorylation of the JAK/Tyk 2 family member, JAK2, which in turn activates and causes nuclear translocation of Stat5a and Stat5b.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Bioworld Technology, Inc (provider)
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- Experimental details
- Western blot (WB) analysis of IL3RA pAb at 1:500 dilutionLane1:MCF-7 whole cell lysateLane2:NIH-3T3 whole cell lysate Lane3:PC12 whole cell lysate