MA5-23818

antibody from Invitrogen Antibodies

Targeting: IL3RA CD123

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Blocking/Neutralizing (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-23818

Provider

Invitrogen Antibodies

Product name

CD123 Monoclonal Antibody (32703)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Reconstitute at 0.5 mg/mL in sterile PBS. Endoxin level is

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

32703

Vial size

100 μg

Concentration

0.5 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of CD123 in immersion fixed human peripheral blood lymphocytes. Samples were incubated in CD123 monoclonal antibody (Product # MA5-23818) using a dilution of 2 µg/mL for 3 hours at room temperature. Cells were stained (red) and counterstained (green).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of CD123 in immersion fixed human peripheral blood lymphocytes. Samples were incubated in CD123 monoclonal antibody (Product # MA5-23818) using a dilution of 2 µg/mL for 3 hours at room temperature. Cells were stained (red) and counterstained (green).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of CD123 in immersion fixed paraffin-embedded sections of human tonsil. Samples were incubated in CD123 monoclonal antibody (Product # MA5-23818) using a dilution of 15 µg/mL overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Neutralization of CD123 in TF‚1 human erythroleukemic cell line. Samples were incubated in CD123 monoclonal antibody (Product # MA5-23818). Recombinant Human IL‚3 stimulates proliferation in the TF‚1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‚3 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‚3 Rα/CD123 Monoclonal Antibody. The ND50 is typically 0.6‚1.2 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Neutralization of CD123 in TF‚1 human erythroleukemic cell line. Samples were incubated in CD123 monoclonal antibody (Product # MA5-23818). Recombinant Human IL‚3 stimulates proliferation in the TF‚1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‚3 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‚3 Rα/CD123 Monoclonal Antibody. The ND50 is typically 0.6‚1.2 µg/mL.