Antibody data
- Antibody Data
- Antigen structure
- References [11]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 11-1239-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD123 Monoclonal Antibody (6H6), FITC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The 6H6 monoclonal antibody reacts with human CD123, the alpha chain of the IL-3 receptor. This 60-70 kDa transmembrane protein binds to IL-3 with low affinity by itself, and when associated with CD131 (common beta chain) binds IL-3 with high affinity. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, and megakaryocytes. Applications Reported: This 6H6 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 6H6 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- 6H6
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Neutrophils Inhibit Synthesis of Mineralized Extracellular Matrix by Human Bone Marrow-Derived Stromal Cells In Vitro.
Group 2 innate lymphoid cell proportions are diminished in young helminth infected children and restored by curative anti-helminthic treatment.
Distinct parameters of the basophil activation test reflect the severity and threshold of allergic reactions to peanut.
Malaria-induced NLRP12/NLRP3-dependent caspase-1 activation mediates inflammation and hypersensitivity to bacterial superinfection.
Frailty in old age is associated with decreased interleukin-12/23 production in response to toll-like receptor ligation.
The activation pattern of blood leukocytes in head and neck squamous cell carcinoma is correlated to survival.
CpG and non-CpG oligodeoxynucleotides directly costimulate mouse and human CD4+ T cells through a TLR9- and MyD88-independent mechanism.
CD56-positive haematological neoplasms of the skin: a multicentre study of the Cutaneous Lymphoma Project Group of the European Organisation for Research and Treatment of Cancer.
Epithelial cells trigger frontline immunoglobulin class switching through a pathway regulated by the inhibitor SLPI.
Local activation of the innate immune system in Buruli ulcer lesions.
Expression of the plasmacytoid dendritic cell marker BDCA-2 supports a spectrum of maturation among CD4+ CD56+ hematodermic neoplasms.
Bastian OW, Croes M, Alblas J, Koenderman L, Leenen LPH, Blokhuis TJ
Frontiers in immunology 2018;9:945
Frontiers in immunology 2018;9:945
Group 2 innate lymphoid cell proportions are diminished in young helminth infected children and restored by curative anti-helminthic treatment.
Nausch N, Appleby LJ, Sparks AM, Midzi N, Mduluza T, Mutapi F
PLoS neglected tropical diseases 2015 Mar;9(3):e0003627
PLoS neglected tropical diseases 2015 Mar;9(3):e0003627
Distinct parameters of the basophil activation test reflect the severity and threshold of allergic reactions to peanut.
Santos AF, Du Toit G, Douiri A, Radulovic S, Stephens A, Turcanu V, Lack G
The Journal of allergy and clinical immunology 2015 Jan;135(1):179-86
The Journal of allergy and clinical immunology 2015 Jan;135(1):179-86
Malaria-induced NLRP12/NLRP3-dependent caspase-1 activation mediates inflammation and hypersensitivity to bacterial superinfection.
Ataide MA, Andrade WA, Zamboni DS, Wang D, Souza Mdo C, Franklin BS, Elian S, Martins FS, Pereira D, Reed G, Fitzgerald KA, Golenbock DT, Gazzinelli RT
PLoS pathogens 2014 Jan;10(1):e1003885
PLoS pathogens 2014 Jan;10(1):e1003885
Frailty in old age is associated with decreased interleukin-12/23 production in response to toll-like receptor ligation.
Compté N, Zouaoui Boudjeltia K, Vanhaeverbeek M, De Breucker S, Tassignon J, Trelcat A, Pepersack T, Goriely S
PloS one 2013;8(6):e65325
PloS one 2013;8(6):e65325
The activation pattern of blood leukocytes in head and neck squamous cell carcinoma is correlated to survival.
Millrud CR, Månsson Kvarnhammar A, Uddman R, Björnsson S, Riesbeck K, Cardell LO
PloS one 2012;7(12):e51120
PloS one 2012;7(12):e51120
CpG and non-CpG oligodeoxynucleotides directly costimulate mouse and human CD4+ T cells through a TLR9- and MyD88-independent mechanism.
Landrigan A, Wong MT, Utz PJ
Journal of immunology (Baltimore, Md. : 1950) 2011 Sep 15;187(6):3033-43
Journal of immunology (Baltimore, Md. : 1950) 2011 Sep 15;187(6):3033-43
CD56-positive haematological neoplasms of the skin: a multicentre study of the Cutaneous Lymphoma Project Group of the European Organisation for Research and Treatment of Cancer.
Assaf C, Gellrich S, Whittaker S, Robson A, Cerroni L, Massone C, Kerl H, Rose C, Chott A, Chimenti S, Hallermann C, Petrella T, Wechsler J, Bagot M, Hummel M, Bullani-Kerl K, Bekkenk MW, Kempf W, Meijer CJ, Willemze R, Sterry W
Journal of clinical pathology 2007 Sep;60(9):981-9
Journal of clinical pathology 2007 Sep;60(9):981-9
Epithelial cells trigger frontline immunoglobulin class switching through a pathway regulated by the inhibitor SLPI.
Xu W, He B, Chiu A, Chadburn A, Shan M, Buldys M, Ding A, Knowles DM, Santini PA, Cerutti A
Nature immunology 2007 Mar;8(3):294-303
Nature immunology 2007 Mar;8(3):294-303
Local activation of the innate immune system in Buruli ulcer lesions.
Peduzzi E, Groeper C, Schütte D, Zajac P, Rondini S, Mensah-Quainoo E, Spagnoli GC, Pluschke G, Daubenberger CA
The Journal of investigative dermatology 2007 Mar;127(3):638-45
The Journal of investigative dermatology 2007 Mar;127(3):638-45
Expression of the plasmacytoid dendritic cell marker BDCA-2 supports a spectrum of maturation among CD4+ CD56+ hematodermic neoplasms.
Jaye DL, Geigerman CM, Herling M, Eastburn K, Waller EK, Jones D
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2006 Dec;19(12):1555-62
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2006 Dec;19(12):1555-62
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD85g (ILT7) APC (Product # 17-5179-42) and Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (left) or Anti-Human CD123 FITC (right). Cells in the monocyte gate and lymphocyte were used for analysis.
- Conjugate
- Green dye