Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
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Validation data
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- Product number
- 10-4027-25 - Provider product page
- Provider
- ABEOMICS Inc.
- Product name
- Anti-IL-3RA Antibody
- Antibody type
- Monoclonal
- Description
- Interleukin-3 receptor subunit alpha (IL-3RA) or CD123 is a high-affinity receptor for interleukin-3 (IL-3), which along with the primary signaling common β (βc) subunit (CD131) forms a heterodimer, enabling cell signaling. IL-3RA chain is a glycoprotein of 360 amino acidic residues, composed by an extracellular domain of 287 amino acid residues, involving a predicted Ig-like domain, two Fn III domains, a transmembrane domain of 30 amino acid residues and by an intracellular domain of 53 residues. It is expressed on the majority of CD34+ hemopoietic progenitors and its expression is rapidly lost during erythroid and megakaryocytic differentiation, moderately decreased during monocytic differentiation and is sustained in the granulocytic lineage. IL-3RA is also expressed in a variety of hematological neoplasms including blastic plasmacytoid dendritic cell neoplasm (BPDCN), acute myeloid leukemia (AML), acute lymphoblastic leukemia (ALL), chronic myeloid leukemia (CML), Hodgkinâs lymphoma and hairy cell leukemia.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
A partial length recombinant protei
n (a.a 120-323) of IL-3RA was use
d as the immunogen for this antibod
y.- Isotype
- IgG
- Antibody clone number
- ABM25C1
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeat freez thawing
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Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Western blot analysis of IL-3RA. Anti-IL-3RA antibody (Clone: ABM25C1) was used at 6 µg/ml on hbrain lysate.
- Protocol
- Protocol
Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Cell surface flow analysis of human IL3-RA in PBMCs (lymphocytes gated) using 0.5 µg/10^6 cells of antibody (Clone: ABM25C1). Green represents isotype control; red represents anti-IL3 RA antibody. Goat anti-mouse PE conjugate was used as secondary antibody. (Cells were incubated with primary antibody for 45 min. then washed twice with PBS by centrifuging at 1100 rpm for 5 min, followed by 30 min incubation with conjugated secondary antibody. Data acquisition was done after washing twice with PBS as mentioned above).
- Protocol
- Protocol