306017

antibody from BioLegend

Targeting: IL3RA CD123

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

306017

Provider

BioLegend

Proper citation

BioLegend Cat#306017, RRID:AB_10900244

Product name

Brilliant Violet 421™ anti-human CD123 [6H6]

Antibody type

Monoclonal

Antigen

Human IL-3Rα transfected COS cells

Description

CD123 is the 70 kD transmembrane α chain of the IL-3 receptor. Alone, CD123 binds IL-3 with low affinity; when CD123 associates with CDw131 (common β chain), it binds IL-3 with high affinity. CD123 does not transduce intracellular signals upon binding IL-3 and requires the β chain for this function. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, megakaryocytes, and some B cells.

Reactivity

Human

Host

Mouse

Conjugate

Blue dye

Isotype

IgG

Vial size

25 tests

Storage

The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References

Submitted references

Basophils and the T helper 2 environment can promote the development of lupus nephritis.

Charles N, Hardwick D, Daugas E, Illei GG, Rivera J
Nature medicine 2010 Jun;16(6):701-7

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Plasmacytoid dendritic cells resident in human thymus drive natural Treg cell development.

Martín-Gayo E, Sierra-Filardi E, Corbí AL, Toribio ML
Blood 2010 Jul 1;115(26):5366-75

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Human peripheral blood leukocytes were stained with HLA-DR FITC and CD123 (clone 6H6) Brilliant Violet 421™ (top) or mouse IgG1 Brilliant Violet 421™ isotype control (bottom). Data shown was gated on lymphocyte population.

Conjugate

Blue dye