Antibody data

Product number
414-1239-42 - Provider product page
Provider
Invitrogen Antibodies
Product name
CD123 Monoclonal Antibody (6H6), Brilliant Violet™ 480, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The 6H6 monoclonal antibody reacts with human CD123, the alpha chain of the IL-3 receptor. This 60-70 kDa transmembrane protein binds to IL-3 with low affinity by itself, and when associated with CD131 (common beta chain) binds IL-3 with high affinity. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, and megakaryocytes. Applications Reported: This 6H6 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 6H6 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Brilliant Violet™ 480 (BV480) is a dye that emits at 479 nm and is intended for use on cytometers equipped with a violet (405 nm) laser. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information. Our internal testing suggests that Brilliant Violet™ 480 (BV480) is not compatible with methanol-based fixation. Excitation: 440 nm; Emission: 479 nm; Laser: Violet Laser. BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen.™
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
6H6
Vial size
100 Tests
Concentration
5 µL/Test
Storage
4°C, store in dark, DO NOT FREEZE!
IL3RA protein structure - 414-1239-42 shown in red.