PA5-28901

antibody from Invitrogen Antibodies

Targeting: MINK1 B55, MAP4K6, MINK, YSK2, ZC3

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-28901

Provider

Invitrogen Antibodies

Product name

MINK1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: HepG2. Predicted reactivity: Mouse (97%), Rat (97%), Zebrafish (80%), Xenopus laevis (85%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Prickle promotes the formation and maintenance of glutamatergic synapses by stabilizing the intercellular planar cell polarity complex.

Ban Y, Yu T, Feng B, Lorenz C, Wang X, Baker C, Zou Y
Science advances 2021 Oct 8;7(41):eabh2974

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Identification of Endogenous Adenomatous Polyposis Coli Interaction Partners and β-Catenin-Independent Targets by Proteomics.

Popow O, Paulo JA, Tatham MH, Volk MS, Rojas-Fernandez A, Loyer N, Newton IP, Januschke J, Haigis KM, Näthke I
Molecular cancer research : MCR 2019 Sep;17(9):1828-1841

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The homophilic receptor PTPRK selectively dephosphorylates multiple junctional regulators to promote cell-cell adhesion.

Fearnley GW, Young KA, Edgar JR, Antrobus R, Hay IM, Liang WC, Martinez-Martin N, Lin W, Deane JE, Sharpe HJ
eLife 2019 Mar 29;8

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MINK1 Polyclonal Antibody detects MAP4K6 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: MAP4K6 protein stained by MINK1 Polyclonal Antibody (Product # PA5-28901) diluted at 1:500. Blue: Hoechst 33342 staining.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 4. Altered composition of synaptic proteins in the PK2 E8Q/E8Q mice. ( A to C ) Western blot analysis of PCP components, regulator of PCP signaling, Mink1, alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor (AMPAR)/N-methyl-D-aspartate receptor (NMDAR) subunits, and MAGUK family proteins in the crude synaptosomes of cortex and HPC from P14 PK2 E8Q/E8Q mice and WT littermates. ( D to F ) Quantitation of protein levels shown in (A) to (C). Each data point represents one animal. Data were normalized by dividing each value to the maximum value in each independent experiment. PK2 +/+ , n = 6; PK2 E8Q/E8Q , n = 6, Mann-Whitney U test, * P < 0.05 and ** P < 0.01. Error bars represent SEM. ( G to L ) Immunofluorescence staining of PK2 and Vangl2 in CA1 SR of P14 mice. To quantify the number (H) and the average size (I) of PK2 puncta, 66 image stacks of CA1 SR were taken from three PK2 +/+ animals, 66 image stacks from three PK2 E8Q/+ animals, and 68 image stacks from three PK2 E8Q/E8Q animals. To quantify the number (K) and the average size (L) of Vangl2 puncta, 62 image stacks were taken from three PK2 +/+ animals, 36 image stacks from two PK2 E8Q/+ animals, and 63 image stacks from PK2 E8Q/E8Q animals. Data were normalized by dividing each value to the maximum value in each independent experiment. Scale bars, 5 mum. Kruskal-Wallis test was used to compare the means of three groups, and Dunn's test was used to perform multiple comparisons. ** P < 0.01, *** P < 0