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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- AF6990 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Glycoprotein V/CD42d Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse Glycoprotein V/CD42d in direct ELISAs and Western blots.
- Reactivity
- Mouse
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
NP_032174- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse Glycoprotein V/CD42d by Western Blot. Western blot shows lysates of mouse platelets. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Glycoprotein V/ CD42d Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6990) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for Glycoprotein V/CD42d at approximately 70-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Glycoprotein V/CD42d in Mouse Platelets. Glycoprotein V/CD42d was detected in immersion fixed mouse platelets using Sheep Anti-Mouse Glycoprotein V/CD42d Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6990) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
