PA5-48292

antibody from Invitrogen Antibodies

Targeting: WTIP

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-48292

Provider

Invitrogen Antibodies

Product name

WTIP Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Predicted to react with mouse based on sequence homology.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

WTIP upregulates FOXO3a and induces apoptosis through PUMA in acute myeloid leukemia.

Zhu Y, Tong X, Wang Y, Lu X
Cell death & disease 2021 Dec 20;13(1):18

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Confocal immunofluorescent analysis of WTIP in WiDr cells. Cells were stained with a WTIP Antibody (C-term) (Product # PA5-48292) followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclei (blue).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 1 Low expression of WTIP correlates with poor prognosis in AML patients. A Predicting the effect of WTIP expression on the survival of AML patients using PROGgeneV2. AML patients were classified into high and low WTIP expression subgroups (as per the median). Kaplan-Meier survival curves showed that patients with low WTIP expression had significantly shorter overall survival. P value was calculated by log-rank test. B Western blot analysis of WTIP expression in the indicated AML cell lines, GAPDH served as a loading control. C Western blot analysis of WTIP expression in mononuclear bone marrow cells derived from 60 AML patients and 17 healthy donors. P values were calculated by non-paired Student's t -test (*** P < 0.001).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 2 Overexpression of WTIP inhibits cell proliferation in AML cells. A Western blot analysis of WTIP expression in KG1a and MOLM-13 cells transfected with WTIP or control vector. B RT-PCR analysis of WTIP mRNA expression in KG1a and MOLM-13 cells transfected with WTIP or control vector. Data are represented as mean +- SD from three independent experiments (*** P < 0.001). C Cell proliferation was detected by MTT assay in KG1a and MOLM-13 cells transfected with WTIP or control vector. Data are represented as mean +- SD from three independent experiments (*** P < 0.001). D MTT assay of KG1a and MOLM-13 cells transfected with WTIP vector shows a decrease in the number of colonies compared with control vector cells. Scale bars, 50 mum. E Colony numbers were calculated and data are represented as mean +- SD from three independent experiments (*** P < 0.001).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 5 FOXO3 gene expression is transcriptionally regulated by WTIP. A MOLM-13 cells transfected with WTIP or control vector were treated with doxycycline for 48 h, and cell lysates were subjected to immunoprecipitation and immunoblotting with anti-WTIP and anti-FOXO3a antibodies, respectively. B FOXO3a-Luc reporter vector was transfected into HEK293 cells, either in the presence of WTIP or control vector, and luciferase activity was measured 48 h after transfection. Data are represented as mean +- SD from three independent experiments (* P < 0.05). C Confocal immunofluorescence analysis of FOXO3a localization in KG1a and MOLM-13 cells transfected with WTIP or control vector. Scale bars, 50 mum.