H00008892-M01

antibody from Abnova Corporation

Targeting: EIF2B2 EIF-2Bbeta, EIF2B

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00008892-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00008892-M01, RRID:AB_425791

Product name

EIF2B2 monoclonal antibody (M01), clone 5B12-E10

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant EIF2B2.

Antigen sequence

MPGSAAKGSELSERIESFVETLKRGGGPRSSEEMA
RETLGLLRQIITDHRWSNAGELMELIRREGRRMTA
AQPSETTVGNMVRRVLKIIREEYGRLHGRTDESDQ
QESLHKLLTSGGLNEDFSFHYAQLQSNIIEAINEL
LVELEGTMENIAAQALEHIHSNEVIMTIGFSRTVE
AFLKEAARKRKFHVIVAECAPFCQGHEMAVNLSKA
GIETTVMTDAAIFAVMSRVNKVIIGTKTILANGAL
RAVTGTHTLALAAKHHSTPLIVCAPMFKLSPQFPN
EEDSFHKFVAPEEVLPFTEGDILEKVSVHCPVFDY
VPPELITLFISNIGGNAPSYIYRLMSELYHPDDHV
L


Isotype

IgG

Antibody clone number

5B12-E10

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

EIF2B2 monoclonal antibody (M01), clone 5B12-E10 Western Blot analysis of EIF2B2 expression in K-562 ( Cat # L009V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

EIF2B2 monoclonal antibody (M01), clone 5B12-E10. Western Blot analysis of EIF2B2 expression in HeLa ( Cat # L013V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of EIF2B2 expression in transfected 293T cell line by EIF2B2 monoclonal antibody (M01), clone 5B12-E10.Lane 1: EIF2B2 transfected lysate(39 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged EIF2B2 is approximately 0.3ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to EIF2B2 on formalin-fixed paraffin-embedded human lymphoma tissue. [antibody concentration 5 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol