PA5-96551

antibody from Invitrogen Antibodies

Targeting: EIF2B2 EIF-2Bbeta, EIF2B

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-96551

Provider

Invitrogen Antibodies

Product name

eIF2b beta Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MPGSAAKGSE LSERIESFVE TLKRGGGPRS SEEMARETLG LLRQIITDHR WSNAGELMEL IRREGRRMTA AQPSETTVGN MVRRVLKIIR EEYGRLHGRS DESDQQESLH KLLTSGGLNE Positive Samples: Mouse thymus, Rat kidney

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.89 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of eIF2b beta in extracts of various cell lines. Samples were incubated with eIF2b beta Polyclonal antibody (Product # PA5-96551) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of eIF2b beta was performed in C6 cells using eIF2b beta Polyclonal Antibody (Product # PA5-96551).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of eIF2b beta was performed in NIH/3T3 cells using eIF2b beta Polyclonal Antibody (Product # PA5-96551).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of eIF2b beta in C6 cells. Samples were incubated with eIF2b beta Polyclonal antibody (Product # PA5-96551) using a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of eIF2b beta in NIH/3T3 cells. Samples were incubated with eIF2b beta Polyclonal antibody (Product # PA5-96551) using a dilution of 1:100. Blue: DAPI for nuclear staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of eIF2b beta in paraffin-embedded rat lung. Samples were incubated with eIF2b beta Polyclonal antibody (Product # PA5-96551) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of eIF2b beta in paraffin-embedded human tonsil. Samples were incubated with eIF2b beta Polyclonal antibody (Product # PA5-96551) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of eIF2b beta in paraffin-embedded mouse brain. Samples were incubated with eIF2b beta Polyclonal antibody (Product # PA5-96551) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of eIF2b beta in paraffin-embedded rat lung. Samples were incubated with eIF2b beta Polyclonal antibody (Product # PA5-96551) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.