NBP1-85639
antibody from Novus Biologicals
Targeting: UBQLN2
Chap1, CHAP1/DSK2, Dsk2, LIC-2, N4BP4, PLIC-2, PLIC2, RIHFB2157
Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [4]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP1-85639 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-85639, RRID:AB_11032720
- Product name
- Rabbit Polyclonal Ubiquilin 2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human Ubiquilin 2 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Mutant UBQLN2 promotes toxicity by modulating intrinsic self-assembly.
Assembly and Function of Heterotypic Ubiquitin Chains in Cell-Cycle and Protein Quality Control.
Differential recruitment of UBQLN2 to nuclear inclusions in the polyglutamine diseases HD and SCA3.
Viral expression of ALS-linked ubiquilin-2 mutants causes inclusion pathology and behavioral deficits in mice.
Tau pathology in frontotemporal lobar degeneration with C9ORF72 hexanucleotide repeat expansion.
Sharkey LM, Safren N, Pithadia AS, Gerson JE, Dulchavsky M, Fischer S, Patel R, Lantis G, Ashraf N, Kim JH, Meliki A, Minakawa EN, Barmada SJ, Ivanova MI, Paulson HL
Proceedings of the National Academy of Sciences of the United States of America 2018 Oct 30;115(44):E10495-E10504
Proceedings of the National Academy of Sciences of the United States of America 2018 Oct 30;115(44):E10495-E10504
Assembly and Function of Heterotypic Ubiquitin Chains in Cell-Cycle and Protein Quality Control.
Yau RG, Doerner K, Castellanos ER, Haakonsen DL, Werner A, Wang N, Yang XW, Martinez-Martin N, Matsumoto ML, Dixit VM, Rape M
Cell 2017 Nov 2;171(4):918-933.e20
Cell 2017 Nov 2;171(4):918-933.e20
Differential recruitment of UBQLN2 to nuclear inclusions in the polyglutamine diseases HD and SCA3.
Zeng L, Wang B, Merillat SA, Minakawa EN, Perkins MD, Ramani B, Tallaksen-Greene SJ, Costa MDC, Albin RL, Paulson HL
Neurobiology of disease 2015 Oct;82:281-288
Neurobiology of disease 2015 Oct;82:281-288
Viral expression of ALS-linked ubiquilin-2 mutants causes inclusion pathology and behavioral deficits in mice.
Ceballos-Diaz C, Rosario AM, Park HJ, Chakrabarty P, Sacino A, Cruz PE, Siemienski Z, Lara N, Moran C, Ravelo N, Golde TE, McFarland NR
Molecular neurodegeneration 2015 Jul 8;10:25
Molecular neurodegeneration 2015 Jul 8;10:25
Tau pathology in frontotemporal lobar degeneration with C9ORF72 hexanucleotide repeat expansion.
Bieniek KF, Murray ME, Rutherford NJ, Castanedes-Casey M, DeJesus-Hernandez M, Liesinger AM, Baker MC, Boylan KB, Rademakers R, Dickson DW
Acta neuropathologica 2013 Feb;125(2):289-302
Acta neuropathologica 2013 Feb;125(2):289-302
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: Ubiquilin 2 Antibody [NBP1-85639] - Simple Western lane view shows a specific band for Ubiquilin 2 in 0.2 mg/ml of RT-4 (Left) and U-251MG (Right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: Ubiquilin 2 Antibody [NBP1-85639] - Electropherogram image(s) of corresponding Simple Western lane view. Ubiquilin 2 antibody was used at 1:20 dilution on RT-4 and U-251MG lysate(s).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Ubiquilin 2 Antibody [NBP1-85639] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-UBQLN2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Ubiquilin 2 Antibody [NBP1-85639] - Staining of human placenta shows moderate to strong cytoplasmic positivity in cytotrophoblast.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Ubiquilin 2 Antibody [NBP1-85639] - Staining of human cerebellum shows moderate to strong cytoplasmic and nuclear positivity in Purkinje cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Ubiquilin 2 Antibody [NBP1-85639] - Staining of human fallopian tube shows weak to moderate cytoplasmic positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Ubiquilin 2 Antibody [NBP1-85639] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neurons and glial cells.