Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [5]
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Validation data
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- Product number
- NBP1-87703 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-87703, RRID:AB_11031441
- Product name
- Rabbit Polyclonal Indoleamine 2,3-dioxygenase/IDO Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human Indoleamine 2,3-dioxygenase/IDO antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Granuloma correlates of protection against tuberculosis and mechanisms of immune modulation by Mycobacterium tuberculosis.
Mehra S, Alvarez X, Didier PJ, Doyle LA, Blanchard JL, Lackner AA, Kaushal D
The Journal of infectious diseases 2013 Apr;207(7):1115-27
The Journal of infectious diseases 2013 Apr;207(7):1115-27
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87703] - Analysis in human cell line EFO-21.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87703] - Analysis in EFO-21 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-IDO1 antibody. Remaining relative intensity is presented. Loading control: anti-PPIB.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87703] - Analysis in control (vector only transfected HEK293T lysate) and IDO1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87703] - Staining of human cerebral cortex shows no cytoplasmic positivity in neuronal cells as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87703] - Staining of human placenta shows moderate to strong cytoplasmic positivity in endothelial cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87703] - Staining of human small intestine shows moderate to strong positivity in lymphoid cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87703] - Staining of human lymph node shows strong positivity in non - germinal center cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87703] - Staining in human placenta and cerebral cortex tissues . Corresponding IDO1 RNA-seq data are presented for the same tissues.