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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Product number
- ABIN350736 - Provider product page
- Provider
- antibodies-online
- Proper citation
- Antibodies-Online Cat#ABIN350736, RRID:AB_10804680
- Product name
- anti-ERK1 / ERK2 antibody
- Antibody type
- Polyclonal
- Antigen
- A synthetic peptide from the phospho T202 & Y204 of human Erk1, Erk2 conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in many species including rat, mouse, zebra fish, xenopus and chicken.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100 μL
- Storage
- Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability.
- Handling
- Avoid freeze and thaw cycles.
Submitted references Proteomics analysis of protein kinases by target class-selective prefractionation and tandem mass spectrometry.
Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer.
Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.
Time-resolved mass spectrometry of tyrosine phosphorylation sites in the epidermal growth factor receptor signaling network reveals dynamic modules.
Molecular cloning, expression, and characterization of the human mitogen-activated protein kinase p44erk1.
Wissing J, Jänsch L, Nimtz M, Dieterich G, Hornberger R, Kéri G, Wehland J, Daub H
Molecular & cellular proteomics : MCP 2007 Mar;6(3):537-47
Molecular & cellular proteomics : MCP 2007 Mar;6(3):537-47
Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer.
Rikova K, Guo A, Zeng Q, Possemato A, Yu J, Haack H, Nardone J, Lee K, Reeves C, Li Y, Hu Y, Tan Z, Stokes M, Sullivan L, Mitchell J, Wetzel R, Macneill J, Ren JM, Yuan J, Bakalarski CE, Villen J, Kornhauser JM, Smith B, Li D, Zhou X, Gygi SP, Gu TL, Polakiewicz RD, Rush J, Comb MJ
Cell 2007 Dec 14;131(6):1190-203
Cell 2007 Dec 14;131(6):1190-203
Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.
Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M
Cell 2006 Nov 3;127(3):635-48
Cell 2006 Nov 3;127(3):635-48
Time-resolved mass spectrometry of tyrosine phosphorylation sites in the epidermal growth factor receptor signaling network reveals dynamic modules.
Zhang Y, Wolf-Yadlin A, Ross PL, Pappin DJ, Rush J, Lauffenburger DA, White FM
Molecular & cellular proteomics : MCP 2005 Sep;4(9):1240-50
Molecular & cellular proteomics : MCP 2005 Sep;4(9):1240-50
Molecular cloning, expression, and characterization of the human mitogen-activated protein kinase p44erk1.
Charest DL, Mordret G, Harder KW, Jirik F, Pelech SL
Molecular and cellular biology 1993 Aug;13(8):4679-90
Molecular and cellular biology 1993 Aug;13(8):4679-90
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