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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [4]
- Other assay [1]
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- Product number
- 21417-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- INPP5J Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: RVGFRHCKD YVAYVRAKHE DVDGNTYQVT FSEESLPKGH GDFILGYYSH NHSILIGITE PFQISLPSSE LASSSTDSSG TSSEGEDDST LELLAPKSRS PSPGKSKRHR SRSPGLARFP GLALRPSSRE RRGASRSPSP QSRRLSRVAP DRSSNGSSRG SSEEGPSGLP GPWAFPPAVP RSLGLLPALR LETVDPGGGG SWGPDREALA PNSLSPSPQG HRGLEEGGLG P (777-1006 aa encoded by BC109288)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.17 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human heart tissue were subjected to SDS PAGE followed by western blot with 21417-1-AP (INPP5J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse lung tissue were subjected to SDS PAGE followed by western blot with 21417-1-AP (INPP5J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol) fixed HeLa cells using 21417-1-AP (INPP5J antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol) fixed HeLa cells using 21417-1-AP (INPP5J antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human heart using 21417-1-AP (INPP5J antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human heart using 21417-1-AP (INPP5J antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney using 21417-1-AP (INPP5J antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney using 21417-1-AP (INPP5J antibody) at dilution of 1:50 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-INPP5J (IP:21417-1-AP, 4ug; Detection:21417-1-AP 1:500) with mouse lung tissue lysate 4000ug.
