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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- PA5-103257 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TAS2R43 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Antibody detects endogenous levels of total TAS2R43.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Human intestinal bitter taste receptors regulate innate immune responses and metabolic regulators in obesity.
Liszt KI, Wang Q, Farhadipour M, Segers A, Thijs T, Nys L, Deleus E, Van der Schueren B, Gerner C, Neuditschko B, Ceulemans LJ, Lannoo M, Tack J, Depoortere I
The Journal of clinical investigation 2022 Feb 1;132(3)
The Journal of clinical investigation 2022 Feb 1;132(3)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TAS2R43 in RAW264.7 cell lysate. Samples were incubated with TAS2R43 polyclonal antibody (Product # PA5-103257).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of TAS2R43 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with TAS2R43 polyclonal antibody (Product # PA5-103257) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Colocalization of TAS2R43 and TAS2R10 with Paneth or goblet cells in primary crypts from patients with obesity. Representative double-immunofluorescence images of jejunal crypts from obese patients. Crypts were stained for TAS2R43 ( A and B ) or TAS2R10 ( C and D ) (red) and alpha-defensin 5 (in Paneth cells) or mucin 2 (in goblet cells) (green). Images in A - C show colocalization, whereas the images in D show a TAS2R10 + cell in close proximity to a goblet cell, but no colocalization. Nuclei were stained with DAPI (blue). Scale bars: 20 mum ( C ) and 50 mum ( A , B , and D ). Each colocalization study was repeated in crypts derived from at least 3 patients with obesity.
