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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- LS-C290071 - Provider product page
- Provider
- LSBio
- Product name
- Anti-ZNF516 Antibody (aa1113-1163) LS-C290071
- Antibody type
- Polyclonal
- Description
- Immunoaffinity purified
- Reactivity
- Human, Guinea Pig
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100µl
- Storage
- Store at 2-8°C for up to 1 year.
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Supportive validation
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- LSBio (provider)
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- Experimental details
- Detection of Human ZNF516 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-ZNF516 antibody used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. ZNF516 was also immunoprecipitated by rabbit anti-ZNF516 antibody BL11715, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
