ABIN1385253

antibody from antibodies-online

Targeting: ZNF516 HsT287, KIAA0222

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

ABIN1385253

Provider

antibodies-online

Product name

anti-Zinc Finger Protein 516 (ZNF516) antibody

Antibody type

Polyclonal

Antigen

KLH conjugated synthetic peptide derived from human ZNF516

Description

purified by Protein A and peptide affinity chromatography

Reactivity

Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Porcine

Host

Rabbit

Isotype

IgG

Vial size

100 μL

Concentration

1 μg/μL

Storage

Store at -20?C for 12 months

Immunohistochemistry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

paraformaldehyde-fixed, paraffin embedded rat testis tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-ZNF516 Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

paraformaldehyde-fixed, paraffin embedded rat testis tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-ZNF516 Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining