PA5-78234

antibody from Invitrogen Antibodies

Targeting: ZFX ZNF926

Western blot

Immunoprecipitation

Antibody data

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Validation data

Product number: PA5-78234 - Provider product page
Provider: Invitrogen Antibodies
Product name: ZFX Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant full-length protein
Description: Positive Control: K562, U87-MG, SK-N-SH, IMR32, SK-N-AS Predicted Reactivity: Mouse (84%), Rat (84%), Bovine (86%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 2.55 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

ZFX protein structure - PA5-78234 shown in red.

Supportive validation

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 4 ZFX and YKL-40 are involved in the HOXA-AS2-miR-302c-3p regulatory axis. a the target site of ZFX and relative miR-302c-3p was confirmed by Target Scan. b , c ZFX and YKL-40 mRNA and proteins levels in endometrial cancer tissues versus normal endometrial tissues measured by qRT-PCR and western blotting. d ZFX and YKL-40 levels in HOXA-AS2-modified Ishikawa cells measured by qRT-PCR and western blotting. e ZFX and YKL-40 levels in miR-302c-3p-modified Ishikawa cells measured by qRT-PCR and western blotting. Data are the mean +- SD from independent experiments. * P < 0.05, ** P < 0.01 *** P < 0.001

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 6 YKL-40 promotes the development of type I endometrial cancer cells and is regulated by ZFX in vitro. a Cell proliferation of YKL-40-modified Ishikawa cells was examined by CCK-8 assay. b Cell cycle phases of YKL-40-modified Ishikawa cells were examined by flow cytometry. c Fractions of apoptotic YKL-40-modified Ishikawa cells were examined by flow cytometry. d Invasion of miR-302c-3p-modified cells was examined by Transwell invasion assays. e , f YKL-40 mRNA and protein levels in ZFX-modified Ishikawa cells were measured by qRT-PCR and western blotting. Data are the mean +- SD from independent experiments. ** P < 0.01, *** P < 0.001. g Proposed negative feedback loop mechanism of the HOXA-AS2-miR-302c-3p/ZFX/YKL-40 axis in Ishikawa cells. HOXA-AS2 regulate the biological behavior of the type I endometrium by negatively regulating miR-302c-3p expression. MiR-302c-3p acts as a tumor suppressor in endometrial cancer, low expression of miR-302c-3p promotes the expression of transcription factor ZFX, and high expression of ZFX promotes the expression of hydrolase YKL-40, which in turn promotes malignant behavior of type I endometrium