AP13557b

antibody from Abcepta

Targeting: RNASE3 ECP, RAF1, RNS3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP13557b

Provider

Abcepta

Proper citation

Abgent Cat#AP13557b, RRID:AB_10892451

Product name

RNASE3 Antibody (C-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

0.47 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

"Western blot analysis of lysate from human spleen tissue lysate, using RNASE3 Antibody (C-term)(Cat. #AP13557b). AP13557b was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 35ug."

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

RNASE3 Antibody (C-term) (Cat. #AP13557b) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the RNASE3 antibody detected the RNASE3 protein (arrow).

Primary Ab dilution

1:1000

Immunocytochemistry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

Confocal immunofluorescent analysis of RNASE3 Antibody (C-term) (Cat#AP13557b) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Primary Ab dilution

1:10~50