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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [2]
- Flow cytometry [1]
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- Product number
- MA5-34931 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RNASE3 Monoclonal Antibody (5G4)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: U937, Hela, SHG-44, human spleen tissue, human kidney tissue.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 5G4
- Vial size
- 100 µL
- Concentration
- 2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Ribonuclease 3 in SHG-44 cells (green). Samples were formalin fixed, permeabilized with 0.1% Triton X-100 in TBS (1 hour, room temperature) and blocked with 1% BSA (15 min, room temperature), incubated with Ribonuclease 3 monoclonal antibody (Product # MA5-34931) at a dilution of 1:50 (1 hour, room temperature), and followed by Alexa Fluor 488 Goat anti-Rabbit IgG and DAPI (blue) with a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Ribonuclease 3 in HeLa cells (green). Samples were formalin fixed, permeabilized with 0.1% Triton X-100 in TBS (1 hour, room temperature) and blocked with 1% BSA (15 min, room temperature), incubated with Ribonuclease 3 monoclonal antibody (Product # MA5-34931) at a dilution of 1:50 (1 hour, room temperature), and followed by Alexa Fluor 488 Goat anti-Rabbit IgG and DAPI (blue) with a dilution of 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Ribonuclease 3 in HeLa cells, (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with Ribonuclease 3 monoclonal antibody (Product # MA5-34931) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-mouse IgG with a dilution of 1:500.
