MA5-34931

antibody from Invitrogen Antibodies

Targeting: RNASE3 ECP, RAF1, RNS3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-34931

Provider

Invitrogen Antibodies

Product name

RNASE3 Monoclonal Antibody (5G4)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Positive Control: U937, Hela, SHG-44, human spleen tissue, human kidney tissue.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

5G4

Vial size

100 µL

Concentration

2 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Ribonuclease 3 in U937 cell lysate. Samples were transferred to PVDF membrane, blocked with 5% BSA (1 hour), incubated with Ribonuclease 3 monoclonal antibody (Product # MA5-34931), at a dilution of 1:500 (5% BSA, 2 hours), followed by Goat Anti-Mouse IgG - HRP (1 hour) with a dilution of 1:5000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Ribonuclease 3 in SHG-44 cells (green). Samples were formalin fixed, permeabilized with 0.1% Triton X-100 in TBS (1 hour, room temperature) and blocked with 1% BSA (15 min, room temperature), incubated with Ribonuclease 3 monoclonal antibody (Product # MA5-34931) at a dilution of 1:50 (1 hour, room temperature), and followed by Alexa Fluor 488 Goat anti-Rabbit IgG and DAPI (blue) with a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Ribonuclease 3 in HeLa cells (green). Samples were formalin fixed, permeabilized with 0.1% Triton X-100 in TBS (1 hour, room temperature) and blocked with 1% BSA (15 min, room temperature), incubated with Ribonuclease 3 monoclonal antibody (Product # MA5-34931) at a dilution of 1:50 (1 hour, room temperature), and followed by Alexa Fluor 488 Goat anti-Rabbit IgG and DAPI (blue) with a dilution of 1:100.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Ribonuclease 3 in paraffin-embedded human spleen tissue. Samples were heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, 20 min) and blocked in 5% BSA (30 min, room temperature), incubated with Ribonuclease 3 monoclonal antibody (Product # MA5-34931) at a dilution of 1:200 (30 min, room temperature), and followed by HRP conjugated compact polymer system, DAB, counterstained with hematoxylin (mounted with DPX).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Ribonuclease 3 in paraffin-embedded human kidney tissue. Samples were heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, 20 min) and blocked in 5% BSA (30 min, room temperature), incubated with Ribonuclease 3 monoclonal antibody (Product # MA5-34931) at a dilution of 1:200 (30 min, room temperature), and followed by HRP conjugated compact polymer system, DAB, counterstained with hematoxylin (mounted with DPX).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of Ribonuclease 3 in HeLa cells, (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with Ribonuclease 3 monoclonal antibody (Product # MA5-34931) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-mouse IgG with a dilution of 1:500.