OSV00047W-100UL

antibody from Invitrogen Antibodies

Targeting: SLC17A8 DFNA25, VGLUT3

Antibody data

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Validation data

Product number: OSV00047W-100UL - Provider product page
Provider: Invitrogen Antibodies
Product name: VGluT3 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Reactivity: Human, Mouse, Rat
Host: Rabbit
Vial size: 100 µL
Concentration: Conc. Not Determined
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.

SLC17A8 protein structure - OSV00047W-100UL shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: IHC-P on paraffin sections of mouse brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 mL 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1:250, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: IHC-P on paraffin sections of mouse brain (hippocampus). The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 mL 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1:250, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: IHC-P on paraffin sections of mouse hippocampus. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 mL 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1:250, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: IHC-P on paraffin sections of mouse hippocampus. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 mL 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1:250, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: IHC-P on paraffin sections of mouse hippocampus. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 mL 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1:250, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: IHC-P on paraffin sections of mouse hippocampus. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 mL 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1:250, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: IHC-P on paraffin sections of mouse hippocampus. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 mL 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1:250, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: IHC-P on paraffin sections of mouse hippocampus. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 mL 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1:250, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: IHC-P on paraffin sections of mouse hippocampus. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 mL 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1:250, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.