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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
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- Product number
- PA5-27554 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SRP14 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Raji.
- Concentration
- 0.73 mg/mL
Submitted references A functional link between the co-translational protein translocation pathway and the UPR.
Plumb R, Zhang ZR, Appathurai S, Mariappan M
eLife 2015 May 20;4
eLife 2015 May 20;4
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SRP14 using 30 µg of Raji lysate. Samples were loaded onto a 15% SDS-PAGE gel and probed with a SRP14 polyclonal antibody (Product # PA5-27554) at a dilution of 1:1500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SRP14 Polyclonal Antibody (Product # PA5-27554). Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with SRP14 Polyclonal Antibody (Product # PA5-27554) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4. SRP mediated targeting to the ER ensures efficient spicing of XBP1u mRNA. ( A ) HEK 293 cells were transfected with shRNAs against luciferase (control), SRP14, or SRP54. 5 days after transfection, the cells were replated for transfection with XBP1u and treated with 10 mM DTT for the indicated time periods. The Trizol harvested cells were analyzed as in Figure 2D . A phos-tag gel was used for the Ire1alpha immunoblot. p-Ire1alpha and p-PERK indicate the phosphorylated forms of Ire1alpha and PERK, respectively. * denotes a background band. ( B ) HeLa cells were transfected with control siRNA or siRNA targeting the alpha subunit of the SRP receptor (SRalpha) and analyzed as described in Figure 2D . ( C ) HeLa cells were transfected with the indicated siRNA oligos and treated with 10 mM DTT for 2 hr after 96 hr post-transfection. Cells were harvested in SDS sample buffer and analyzed for IB with indicated antibodies. Upon DTT treatment, the ATF6alpha band disappears due to the cleavage of its N-terminal cytosolic domain. Our ATF6alpha antibodies were not suitable for detecting the cleaved N-terminal cytosolic domain (not shown). Note that depletion of Sec61alpha caused significant reduction of the transmembrane proteins PERK and ATF6alpha. DOI: http://dx.doi.org/
