HPA024142

antibody from Atlas Antibodies

Targeting: USP25 USP21

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA024142

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA024142, RRID:AB_1858673

Product name

Anti-USP25

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

YRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQ
NNKELLSKGLYRGHDEELISHYRRECLLKLNEQAA
ELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEME
EKDILAVEDMRNRWCSYLGQEMEPHLQEKLTDFLP
KLLDCSMEIK


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Recombinant expression validation

Main image

Experimental details

Western blot analysis in control (vector only transfected HEK293T lysate) and USP25 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY415616).

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human pancreas shows cytoplasmic positivity in exocrine glandular cells and islets of Langerhans.

Sample type

HUMAN