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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- LS-C99571 - Provider product page
- Provider
- LSBio
- Proper citation
- LifeSpan Cat#LS-C99571, RRID:AB_2212888
- Product name
- USP25 Antibody (aa1037-1068) LS-C99571
- Antibody type
- Polyclonal
- Description
- Ammonium sulfate precipitation
- Reactivity
- Human, Rat
- Host
- Rabbit
- Storage
- Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- The anti-USP25 C-term antibody is used in Western blot to detect USP25 in rat testis tissue lysate (lane 1) and USP25-transfected HeLa cell lysates (lane 2). Transfection data is kindly provided by Dr. B. Pierrat from the Novartis Institute for Biomedical Research (Basel, Switzerland).
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
