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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- PA5-96884 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- USP25 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Sequence of this protein is as follows: MTLHEPANSS ASQSTDLCDF SGDLDPAPNP PHFPSHVVKA TFAYISNCHK TKLKSILEIL SKSPDSYQKI LLAICEQAAE TNNVYKKHRI LKIYHLFVSL LLKDIKSGLG GAWAFVLRDV IYTLIHYINQ RKLTIFSQ
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.52 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of USP25 in extracts of various cell lines using USP25 Polyclonal Antibody (Product # PA5-96884) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of USP25 in paraffin-embedded mouse heart using USP25 Polyclonal Antibody (Product # PA5-96884) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of USP25 in paraffin-embedded mouse brain using USP25 Polyclonal Antibody (Product # PA5-96884) at a dilution of 1:200.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of USP25 was performed in 200 µg extracts of HeLa cells using USP25 Polyclonal Antibody (Product # PA5-96884). Western blot was performed from the immunoprecipitate using USP25 Polyclonal Antibody.
