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- Antibody Data
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- RQ6017 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- USP25 Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific USP25 antibody is suitable for use in Western blot/Immunofluorescence/Flow cytometry/Direct ELISA applications with human, mouse and rat, monkey samples.
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the USP25 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human HEK293, 2) human placenta, 3) monkey liver, 4) monkey kidney, 5) human PC-3, 6) human HeLa, 7) human A549, 8) rat kidney, 9) rat liver, 10) rat lung, 11) rat PC-12, 12) mouse lung, 13) mouse RAW264.7 and 14) mouse NIH 3T3 lysate with USP25 antibody. Predicted molecular weight ~122 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human A549 cells with USP25 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human HL60 cells with USP25 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= USP25 antibody.
