GTX15842
antibody from GeneTex
Targeting: PECAM1
CD31
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Flow cytometry [3]
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Validation data
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- Product number
- GTX15842 - Provider product page
- Provider
- GeneTex
- Product name
- CD31 antibody [HEC7]
- Antibody type
- Monoclonal
- Reactivity
- Human, Rat, Bovine
- Host
- Mouse
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of CD31 (green) in THP-1 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with CD31/PECAM-1 antibody [HEC7] in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4¢XC in a humidified chamber. Cells were washed with PBST and incubated with a proper secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Flow cytometry analysis of CD31 in BAEC cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with CD31/PECAM-1 antibody [HEC7] at a dilution of 0.5 ug/test for 60 min at room temperature. Cells were then incubated in a proper secondary antibody and re-suspended in PBS for FACS analysis.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- FACS analysis of PBMC cells using CD31 antibody [HEC7] (green) or an isotype control (blue).
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- FACS analysis of PBMC cells using CD31 antibody [HEC7].