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- References [1]
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- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- AM32708PU-N - Provider product page
- Provider
- OriGene
- Product name
- CD31 (PECAM1) mouse monoclonal antibody, clone BC16-6.4EF, Purified
- Antibody type
- Monoclonal
- Description
- CD31 (PECAM1) mouse monoclonal antibody, clone BC16-6.4EF, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- PECAM1
- Isotype
- IgG
- Antibody clone number
- BC16-6.4EF
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
Submitted references Tyrosinase Is a Novel Endogenous Regulator of Developmental and Inflammatory Lymphangiogenesis.
Büttner C, Clahsen T, Regenfuss B, Dreisow ML, Steiber Z, Bock F, Reis A, Cursiefen C
The American journal of pathology 2019 Feb;189(2):440-448
The American journal of pathology 2019 Feb;189(2):440-448
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blotting with anti CD31/PECAM1 Antibody ?(Clone BC16-6.4EF) used at 2 ug/ml (in Blotto) on HUVEC cell lysate loaded?at 10, 5 and 2.5 ug/lane.? Secondary Antibody: Peroxidase conjugated Rabbit anti-Mouse Ig used at 1/10,000 dilution in?Blotto. Developed by enhanced?chemiluminescence.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Human Umbilical Vein Endothelial Cells (HUVEC) stained with 5 ug/ml CD31/PECAM1 Antibody (Clone BC16-6.4EF)?diluted in PBS with 1% BSA. FITC conjugated Rabbit anti Mouse Ig secondary.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Staining of FFPE Human colon cancer (10x and 40x) with CD31/PECAM1 Antibody (Clone BC16-6.4EF) at 2 ug/ml. Antibody positive in cytoplasm of interstitial endotelial cells modulating neoangiogenesis.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- CD31/PECAM1 Antibody ?(Clone BC16-6.4EF) staining of the vasculature?in small round cell tumors. Indirect immunoperoxidase?staining with Peroxidase conjugated anti-Mouse Ig secondary?antibody. DAB substrate, hematoxylin counterstain.
- Validation comment
- IHC
