A01513-2

antibody from Boster Biological Technology

Targeting: PECAM1 CD31

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

A01513-2

Provider

Boster Biological Technology

Product name

Anti-CD31/Pecam1 Antibody Picoband™

Antibody type

Polyclonal

Description

Rabbit IgG polyclonal antibody for CD31/Pecam1 detection. Tested with WB, FCM, Direct ELISA in Mouse;Rat.

Reactivity

Mouse, Rat

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

References

Submitted references

G-Quadruplex/Hemin DNAzyme-Functionalized Silver Nanoclusters with Synergistic Antibacterial and Wound Healing Capabilities for Infected Wound Management.

Zhu J, Wen T, Qu S, Li Q, Liu B, Zhou W
Small (Weinheim an der Bergstrasse, Germany) 2024 Feb;20(8):e2307220

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EPAC inhibitor suppresses angiogenesis and tumor growth of triple-negative breast cancer.

Li Z, Liu Q, Cai Y, Ye N, He Z, Yao Y, Ding Y, Wang P, Qi C, Zheng L, Wang L, Zhou J, Zhang QQ
Biochimica et biophysica acta. Molecular basis of disease 2024 Apr;1870(4):167114

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Exosomes Derived from E2F1(-/-) Adipose-Derived Stem Cells Promote Skin Wound Healing via miR-130b-5p/TGFBR3 Axis.

Yu H, Wu Y, Zhang B, Xiong M, Yi Y, Zhang Q, Wu M
International journal of nanomedicine 2023;18:6275-6292

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Antibacterial, conductive nanocomposite hydrogel based on dextran, carboxymethyl chitosan and chitosan oligosaccharide for diabetic wound therapy and health monitoring.

Zhao N, Yuan W
International journal of biological macromolecules 2023 Dec 31;253(Pt 1):126625

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Angiogenesis-Browning Interplay Mediated by Asprosin-Knockout Contributes to Weight Loss in Mice with Obesity.

Yin T, Chen S, Zeng G, Yuan W, Lu Y, Zhang Y, Huang Q, Xiong X, Xu B, Huang Q
International journal of molecular sciences 2022 Dec 18;23(24)

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Performance of a multilayered small-diameter vascular scaffold dual-loaded with VEGF and PDGF.

Han F, Jia X, Dai D, Yang X, Zhao J, Zhao Y, Fan Y, Yuan X
Biomaterials 2013 Oct;34(30):7302-13

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of Pecam1 using anti-Pecam1 antibody (A01513-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Pecam1 antigen affinity purified polyclonal antibody (Catalog # A01513-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Pecam1 at approximately 120-130KD. The expected band size for Pecam1 is at 82KD.

Additional image