Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunohistochemistry [14]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- UM500106CF - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PECAM1 Monoclonal Antibody (UMAB164), UltraMAB™
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- UMAB164
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot of cell lysates (35 µg) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549, 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7). Dilution: 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of extracts (35 µg) from human kidney tissue by using anti-PECAM1 monoclonal antibody. 1:500
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- Invitrogen Antibodies (provider)
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- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PECAM1 (RC208654, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-PECAM1 mouse monoclonal antibody.1:500`
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of cell lysates (35 µg) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549, 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7). Dilution: 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35 µg) from human kidney tissue by using anti-PECAM1 monoclonal antibody. 1:500
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PECAM1 mouse monoclonal antibody. (UM500106, heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemical staining of paraffin-embedded human placenta using anti-PECAM clone UMAB164 mouse monoclonal antibody (UM500106) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer using pressure chamber for 3 minutes at 110°C. Cytoplasmic and membraneous staining is seen in the endothelia cells and weak staining in the trophoblast.
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemical staining of paraffin-embedded human skin using anti-PECAM clone UMAB164 mouse monoclonal antibody (UM500106) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer using pressure chamber for 3 minutes at 110°C. Cytoplasmic and membraneous staining is seen in the vasular endothelia cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PECAM1 mouse monoclonal antibody. (UM500106, heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human pancreas tissue using anti-PECAM1 mouse monoclonal antibody. (UM500106, heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PECAM1 mouse monoclonal antibody. (UM500106, heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PECAM1 mouse monoclonal antibody. (UM500106, heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human endometrium tissue using anti-PECAM1 mouse monoclonal antibody. (UM500106, heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human prostate tissue using anti-PECAM1 mouse monoclonal antibody. (UM500106, heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human bladder tissue using anti-PECAM1 mouse monoclonal antibody. (UM500106, heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PECAM1 mouse monoclonal antibody. (UM500106, heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human tonsil using anti-PECAM1 mouse monoclonal antibody. (UM500106, heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PECAM1 mouse monoclonal antibody. (UM500106, heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PECAM1 mouse monoclonal antibody. (UM500106, heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)
Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Living HEK293T cells transfected with either RC208654 plasmid (red) or empty vector (blue) were immunostained by anti-PECAM1 antibody (UM500106) or isotype control antibody (green), and then analyzed by flow cytometry. (1:100)
Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-PECAM1 mouse monoclonal antibody (UM500106). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.