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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Immunocytochemistry [3]
- Immunohistochemistry [1]
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- Product number
- PA5-101536 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- C1QL2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Antibody detects endogenous levels of total C1QL2.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 μL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Anterior thalamic dysfunction underlies cognitive deficits in a subset of neuropsychiatric disease models.
Roy DS, Zhang Y, Aida T, Choi S, Chen Q, Hou Y, Lea NE, Skaggs KM, Quay JC, Liew M, Maisano H, Le V, Jones C, Xu J, Kong D, Sullivan HA, Saunders A, McCarroll SA, Wickersham IR, Feng G
Neuron 2021 Aug 18;109(16):2590-2603.e13
Neuron 2021 Aug 18;109(16):2590-2603.e13
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of C1QL2 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with C1QL2 polyclonal antibody (Product # PA5-101536) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of C1QL2 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with C1QL2 polyclonal antibody (Product # PA5-101536) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of C1QL2 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with C1QL2 polyclonal antibody (Product # PA5-101536) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of C1QL2 in human ovarian cancer. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with C1QL2 polyclonal antibody (Product # PA5-101536) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
