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- Antigen structure
- References [1]
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- Product number
- PA5-102549 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MT2A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Antibody detects endogenous levels of total MT2A.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Fatty Acid Uptake in Liver Hepatocytes Induces Relocalization and Sequestration of Intracellular Copper.
Harder NHO, Lee HP, Flood VJ, San Juan JA, Gillette SK, Heffern MC
Frontiers in molecular biosciences 2022;9:863296
Frontiers in molecular biosciences 2022;9:863296
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- FIGURE 3 Palmitate stimulations induce changes in cytosolic markers of copper status. Analysis of copper chaperones in the lysates of cells treated with PA or BSA for 1, 6, 12, and 24 h. (A) Representative western blot images of Mt2A. (B) Gene expression analysis of SOD1 ( n = 10) and Mt2A ( n = 10) of PA-stimulated cells relative to BSA-stimulated cells at the same time points (normalized to beta-actin as the housekeeping gene). The Mann-Whitney U test was used to assess the statistical significance. (* p < 0.05 and ** p < 0.01). Mean +- SEM is shown. (C) Ratio of oxidized-to-total glutathione in PA-stimulated cells. The unpaired Student's t -test was used to assess significance.
