600-401-J12

antibody from Invitrogen Antibodies

Targeting: ARHGAP22 RhoGAP2

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

600-401-J12

Provider

Invitrogen Antibodies

Product name

Phospho-Arhgap22 (Ser397) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

50 µg

Concentration

1.07 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot of Rabbit anti-ARHGAP22 pS397 antibody. Lane 1: NIH3T3 cells transfected with a null vector. Lane 2: NIH3T3 cells transfected with ARHGAP22. Lane 3: NIH3T3 cells transfected with ARHGAP22 S22 to alanine mutation. Lane 4: NIH3T3 cells transfected with ARHGAP22 S397 to alanine mutation. Primary antibody: Left: ARHGAP22 pS22, Right: ARHGAP22 pS397 antibody at 1µg/mL for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO O/N at 4°C. Predicted/Observed size: 68 kDa for ARHGAP22. Other band(s): Unmodified ARHGAP22. ARHGAP22-pS22 antibody recognizes the S397>A mutation, not the S22>mutation; ARHGAP22 pS397 recognizes the pS22>A mutation, not the pS397>A mutation; Confirms the specificity of each ARHGAP22 phospho specific antibody.