PA5-21826

antibody from Invitrogen Antibodies

Targeting: TUBB6 HsT1601, MGC4083

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-21826

Provider

Invitrogen Antibodies

Product name

beta Tubulin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji, NIH-3T3.

Concentration

1 mg/mL

References

Submitted references

Therapeutic efficacy of a novel βIII/βIV-tubulin inhibitor (VERU-111) in pancreatic cancer.

Kashyap VK, Wang Q, Setua S, Nagesh PKB, Chauhan N, Kumari S, Chowdhury P, Miller DD, Yallapu MM, Li W, Jaggi M, Hafeez BB, Chauhan SC
Journal of experimental & clinical cancer research : CR 2019 Jan 23;38(1):29

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using beta Tubulin Polyclonal Antibody (Product # PA5-21826). Sample (30 µg whole cell lysate). A: A431. B: Hep G2. 10% SDS PAGE. Beta Tubulin Polyclonal Antibody (Product # PA5-21826) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using beta Tubulin Polyclonal Antibody (Product # PA5-21826). Sample (30 µg of whole cell lysate). Lane A: NIH-3T3. 10% SDS PAGE. beta Tubulin Polyclonal Antibody (Product # PA5-21826) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-beta Tubulin Rabbit Polyclonal Antibody (Product # PA5-21826) and a 52kDa band corresponding to beta Tubulin was observed across cell lines tested. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), NIH-3T3 (Lane 2) and PC-12 (Lane 3) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of beta Tubulin was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: beta Tubulin Polyclonal Antibody (Product # PA5 21826) diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of beta Tubulin was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with beta Tubulin Polyclonal Antibody (Product # PA5-21826) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing microtubular localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.