Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- MA1-34966 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Calpain S1/S2 Monoclonal Antibody (28F3)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- This antibody specifically recognizes the 28 to 30 kD small subunit of Calpain 1 and Calpain 2 (mu calpain and m calpain). It recognizes both native and denatured protein in ELISA, Western Blot and Immunoprecipitation.
- Reactivity
- Human, Bovine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 28F3
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot was performed using Anti-Calpain S1/S2 Monoclonal Antibody (28F3) (Product # MA1-34966) and a 28kDa band corresponding to Calpain S1/S2 was observed across the tested cell lines, except Jurkat and NIH/3T3. Whole Cell Extract-TEB (30 µg lysate) of MCF7 (Lane 1), IMR-32 (Lane 2), Jurkat (Lane 3), DU 145 (Lane 4), NIH/3T3 (Lane 5), A-431 (Lane 6), and PC-3 (Lane 7) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).