Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF2547 - Provider product page
- Provider
- R&D Systems
- Product name
- Human FGF-20 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human FGF-20 in direct ELISAs and Western blots. In direct ELISAs, less than 40% cross-reactivity with recombinant human (rh) FGF-16 and rhFGF-9 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q9NP95
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Fgf20 is required for sensory epithelial specification in the developing cochlea.
Hayashi T, Ray CA, Bermingham-McDonogh O
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Jun 4;28(23):5991-9
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Jun 4;28(23):5991-9
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by FGF-20 and Neutralization by Human FGF-20 Antibody. Recombinant Human FGF-20 (Catalog # 2547-FG) stimulates proliferation in the the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human FGF-20 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human FGF-20 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2547). The ND50 is typically 0.05-0.35 µg/mL in the presence of heparin (1 µg/mL).